DDC Technology Day | An Improved Method for Determining Fumaric Acid Content in Acidifiers Using High-Performance Liquid Chromatography

Pick    To: High-performance liquid chromatography analysis of fumaric acid. United States Zorbax Eclipse XDB-C18 ( 4.6 × 150 mm ，5 um ); 0.50% Phosphoric acid - Distilled water 1：9 , volume ratio) eluting solvent, flow rate 1 mL/min ; Column temperature 30 ℃; Detection Wavelength 210 nm The average recovery rate of fumaric acid is 98.67% ， RSD = 1.26% ( n=5 ). This method is simple, offers rapid peak detection, and can serve as a new, highly efficient HPLC-based analytical approach for fumaric acid in feed.

Keywords: Fumaric acid, high-performance liquid chromatography

Fumaric acid Fumaric acid ) Also known as maleic acid, violuric acid, and lichen acid, Fumaric acid It is typically a white crystalline powder, slightly soluble in cold water, with a fruity acidic taste. Its chemical formula is C ₄ H ₄ O ₄ , is an important organic chemical raw material and fine chemical product ^[1] Currently, the main methods for determining fumaric acid include potentiometric titration. ^[2] , Spectrophotometry ^[3] , Ion Exchange Chromatography ^[4] And High-Performance Liquid Chromatography Method ^[5-8] And so on. In recent years, studies determining fumaric acid have primarily focused on simpler matrices such as reference standards, wastewater, fermentation broth, wine, and fruit juices—while reports on the quantification of fumaric acid in feed acidifiers remain scarce. This paper presents a novel method for the determination of fumaric acid in feed acidifiers. HPLC The detection method demonstrates excellent repeatability and stability, enabling faster and more accurate determination of fumaric acid content.

Detailed link: https://Click Here